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Messages - Blonic the Fluxmeister
Ended up getting a 3.93. Could've had a 4.0 if I didn't fuck up one exam in one of my classes.
« on: December 27, 2016, 11:08:21 AM »
So is ONI destabilizing the Middle East?
kill yourself my man
People have claims that you kicked everyone. The burden of proof rests on you.proof I didn't do something lmaoGonna need to see some proofs for that.this never happened but okClass kicked everyone, and was the only mod online at the time it happened, and is now poorly trying to make it seem like he did nothing and that everyone should join the server where he is now admin to sate his ego among other things I imagine.Is this the sperg out I heard about?
Gonna need to see some proofs for that.this never happened but okClass kicked everyone, and was the only mod online at the time it happened, and is now poorly trying to make it seem like he did nothing and that everyone should join the server where he is now admin to sate his ego among other things I imagine.Is this the sperg out I heard about?
« on: November 25, 2016, 10:49:56 AM »
So...you don't like good music?
Is this the Sep7 one or the nazi oneHeil fucking Hitler.
Leppo my eppo?>What is the checks and balances system.What is Aleppo?
« on: October 31, 2016, 09:28:31 PM »
Voting for Trump. :3
« on: October 11, 2016, 07:13:26 AM »
ITT: Flee is fukt.
« on: October 01, 2016, 08:19:48 PM »
Addictinggames.com was my childhood, so fuck off.
it's rampant in our society and desperately needs to be stoppedI know right? Muslims are so very anti-semitic.
« on: October 01, 2016, 12:57:48 PM »
>he doesn't get high off of Flintstone's gummies
hashtag dadbodI think that stash makes you look like a pedo.
From what I know, I don't think anyone has tried using ZFNs and TALENs in human embryos like that china paper, considering that gene editing in humans is a rather controversial topic. Maybe there are other papers that have done a similar approach with these other technologies, but I'm not entirely sure. Most research using these technologies has been in other model organisms.There was an article published in 2015, and was mentioned late in the first video, by scientists in china who used CRISPR/Cas9 on tripronuclear (3PN) human zygotes to see if they could effectively repair a defective copy of a certain gene. However, the frequency of homologous recombination of their supplied transcript was low, roughly 25% (off the top of my head).
And yes, I agree with the idea that is can be a very innovative technology down the road, but I felt that the video was overblowing its current capabilities and really tending to shrug off the technical issues that CRISPR faces. CRISPR is definitely a cheaper and faster method for genome editing, but it also lacks the specificity of ZFNs and TALENs.
It's gonna be quite a while for sure before we really see full-fledged genome editing in humans. The CRISPR/Cas9 technology, which has been adopted from bacteria, is still very much in its infancy and has many complications. The reason why it is heralded as being such a game changer is that this technology functions much differently as opposed to older genome editing technologies like Zinc-Finger Nucleases (ZFNs and Transcription activator-like effector nucleases (TALENs). These earlier technologies involved altering the binding specificity of the DNA-binding domains to bind to specific DNA sequences, which took time to develop. With CRISPR, on the other hand, all people need to do is synthesize a guide RNA (gRNA) that binds to a specific DNA sequence and the CRISPR/Cas9 will do its job.
However, just like earlier genome editing techniques, CRISPR is used to created double stranded breaks at a target sequence. This can be used to knockout genes, introduce transgenes through the homology-directed repair pathway, or repair defective copies of genes by supplying a normal copy of the gene that gets incorporated through the HDR mechanism. There's a few other uses for it, but those three are generally the most common uses.
Like I said before, this technology is in its infancy and still has many issues. One of the biggest issues is that CRISPR/Cas9 has a greater likelihood of off-target effects opposed to TALENs and ZFNs. There was an article published in 2015, and was mentioned late in the first video, by scientists in china who used CRISPR/Cas9 on tripronuclear (3PN) human zygotes to see if they could effectively repair a defective copy of a certain gene. However, the frequency of homologous recombination of their supplied transcript was low, roughly 25% (off the top of my head). Even then, there were issues of CRISPR/Cas9 having off-target endonuclease activity on a homolog for the gene, which they were unable to resolve.
I think the first video really over-glorifies the capabilities of CRISPR/Cas9 as it is now, but I am rather interested in its gene drive implications that appear to lead to "super-mendelian inheritance". There is also research into combining the sequence targeting capabilities of CRISPR with enzymatic domains of adenosine deaminases to create base editors that appear to have more effective use compared to the standard CRISPR/Cas9 system.
Also, no TL;DR, you lazy fucks.
« on: August 06, 2016, 08:22:15 PM »
SecondClass because he is a nu-male cuck apparently.
« on: August 06, 2016, 02:39:03 PM »
Idk, haven't really read any research papers on that stuff.No, not really. It's just people overeating. Some researchers tested some mice that were ob/ob, meaning they had homozygous mutation that promoted obesity. What they found was that a hypoediting of a sodium channel transcript at one specific location led to the mice overeating. In an environment where the mice could at ad libitum, the mice became obese. However, when the eating habits of the mice were controlled, they were a normal weight compared to normal mice under the same regimen. In other words, fatties are lazy fucks that don't know what self-control is and want to blame other people for their faults.I'm still trying to figure out how people can maintain being over 300+ lbs. Like, just how much crap are people eating to be like 300+ lbs? The biggest I ever got to was 210 lbs, but I've gotten myself down to 153 lbs. Honestly, people these days don't like to take responsibility for their actions. This is why people always claim "muh genetics" when in reality they are just fucking pigs. Do people not realize how easy it is to lose weight when they stop drinking soda?