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Messages - Blonic the Fluxmeister

Pages: 123 45 ... 65
61
The Flood / ITT: Post your grades/GPA for the semester.
« on: December 28, 2016, 01:56:11 PM »
Ended up getting a 3.93.  Could've had a 4.0 if I didn't fuck up one exam in one of my classes.

62
Serious / Re: UNSC condemns Israeli settlements - US abstained from vote
« on: December 27, 2016, 11:08:21 AM »
So is ONI destabilizing the Middle East?

63
The Flood / Re: Apparently I have "white privilege"
« on: December 27, 2016, 10:58:56 AM »
White privilege is a buzzword invented to disrupt discussion.  It's pretty sad tbh.

"Oh, you're white, therefore anything you say is invalid and I automatically win the argument. *furious snapping*"

64
The Flood / Re: Discord server has been moved here
« on: November 27, 2016, 07:52:32 PM »
YouTube


This is now a "We are Number One" thread.
kill yourself my man

literal fucking piece of shit
YouTube

65
The Flood / Re: Discord server has been moved here
« on: November 27, 2016, 12:33:00 PM »
YouTube


This is now a "We are Number One" thread.

66
The Flood / Re: Discord server has been moved here
« on: November 27, 2016, 01:20:55 AM »
Is this the sperg out I heard about?

What sperg out? The fuck is going on?
Class kicked everyone, and was the only mod online at the time it happened, and is now poorly trying to make it seem like he did nothing and that everyone should join the server where he is now admin to sate his ego among other things I imagine.
this never happened but ok
Gonna need to see some proofs for that.
proof I didn't do something lmao
People have claims that you kicked everyone.  The burden of proof rests on you.

67
The Flood / Re: Discord server has been moved here
« on: November 27, 2016, 01:15:06 AM »
Is this the sperg out I heard about?

What sperg out? The fuck is going on?
Class kicked everyone, and was the only mod online at the time it happened, and is now poorly trying to make it seem like he did nothing and that everyone should join the server where he is now admin to sate his ego among other things I imagine.
this never happened but ok
Gonna need to see some proofs for that.

68
So...you don't like good music?

69
The Flood / Re: Discord voice chat 11/21 (now)
« on: November 22, 2016, 09:52:48 AM »
Is this the Sep7 one or the nazi one
Heil fucking Hitler.

70
The Flood / Re: I don't care about GMOs
« on: November 22, 2016, 09:51:56 AM »
So you aren't as vehement about trump and right-leaning individuals as you made yourself out to be in the discord chats?
I'm confuzzled.

71
The Flood / Happy birthday Deci.
« on: November 17, 2016, 03:39:06 PM »
You're just one year closer to your death, thank god.

72
The Flood / Re: what a disgusting fucked up society
« on: November 11, 2016, 05:27:51 PM »

73
The Flood / Re: what a disgusting fucked up society
« on: November 11, 2016, 05:21:23 PM »
>What is the checks and balances system.

74
Serious / Re: President Elect Donald J Trump
« on: November 09, 2016, 12:42:46 AM »
Pennsylvania was called for Trump.  It's official, Trump wins.

75
Serious / Re: President Elect Donald J Trump
« on: November 09, 2016, 12:20:10 AM »
I bet SecondClass is crying as he watches Clinton get fucked.

76
Serious / Re: "Voter fraud doesn't happen in the US!"
« on: October 31, 2016, 10:11:40 PM »
Don't forget the video of the DNC chairman admitting that voter fraud occurs, especially from the left.

77
Voting for Trump.  :3

78
The Flood / Re: At the airport, heading to Warsaw for a convention
« on: October 11, 2016, 07:13:26 AM »
ITT: Flee is fukt.

79
Serious / Re: Debate Number 2
« on: October 09, 2016, 09:15:23 PM »
Talk about a biased moderator.

80
Addictinggames.com was my childhood, so fuck off.

81
Serious / Re: Anti Semitism
« on: October 01, 2016, 12:59:50 PM »
it's rampant in our society and desperately needs to be stopped
I know right?  Muslims are so very anti-semitic.

82
>he doesn't get high off of Flintstone's gummies

sheeeeeeit

83
The Flood / Re: Is This Place Worth Returning To?
« on: September 27, 2016, 05:22:56 PM »
Go take your stupid Rubicon elsewhere, Chakas.  The Lifeshaper isn't here.

84
The Flood / Re: mirror selfie thread
« on: September 27, 2016, 03:00:11 PM »
hashtag dadbod
Spoiler

I'll still fuck up every single one of you in a fight.
Except maybe Jive 'cause he's a juicing ass nigga now
I think that stash makes you look like a pedo.

85
Serious / Re: Genetic Engineering, Pt. II - CRISPR & CAS9
« on: September 27, 2016, 06:27:18 AM »
By the way, if anyone is actually interested in reading some good review/research articles on CRISPR, I'm happy to provide them.

86
Serious / Re: Genetic Engineering, Pt. II - CRISPR & CAS9
« on: September 26, 2016, 12:30:20 PM »
There was an article published in 2015, and was mentioned late in the first video, by scientists in china who used CRISPR/Cas9 on tripronuclear (3PN) human zygotes to see if they could effectively repair a defective copy of a certain gene.  However, the frequency of homologous recombination of their supplied transcript was low, roughly 25% (off the top of my head).

Is 25% success higher than what we're currently getting with ZFN or TALEN methods? If not, then the main advantage of CRISPR is saving time and funding to produce at least some results (I'm thinking a quick brute-force method over meticulous but productive).

Quote
I think the first video really over-glorifies the capabilities of CRISPR/Cas9 as it is now, but I am rather interested in its gene drive implications that appear to lead to "super-mendelian inheritance".  There is also research into combining the sequence targeting capabilities of CRISPR with enzymatic domains of adenosine deaminases to create base editors that appear to have more effective use compared to the standard CRISPR/Cas9 system.

I kinda got that vibe too from it, but if it's something that could be spectacular in it's infancy then it's worth waiting to see what comes of it as it's researched and tested more (particularly if the upfront cost is lower).

Who knows, with gene-drive it could be possible to eradicate other diseases by targeting the parents without having to genetically alter a zygote/fetus during pregnancy and all the risks that brings (but I'm completely out of my element here, so I don't know how exactly that would work, if at all).
From what I know, I don't think anyone has tried using ZFNs and TALENs in human embryos like that china paper, considering that gene editing in humans is a rather controversial topic.  Maybe there are other papers that have done a similar approach with these other technologies, but I'm not entirely sure.  Most research using these technologies has been in other model organisms.

  And yes, I agree with the idea that is can be a very innovative technology down the road, but I felt that the video was overblowing its current capabilities and really tending to shrug off the technical issues that CRISPR faces.  CRISPR is definitely a cheaper and faster method for genome editing, but it also lacks the specificity of ZFNs and TALENs.

87
Serious / Re: Genetic Engineering, Pt. II - CRISPR & CAS9
« on: September 26, 2016, 11:01:52 AM »
It's gonna be quite a while for sure before we really see full-fledged genome editing in humans.  The CRISPR/Cas9 technology, which has been adopted from bacteria, is still very much in its infancy and has many complications.  The reason why it is heralded as being such a game changer is that this technology functions much differently as opposed to older genome editing technologies like Zinc-Finger Nucleases (ZFNs and Transcription activator-like effector nucleases (TALENs).  These earlier technologies involved altering the binding specificity of the DNA-binding domains to bind to specific DNA sequences, which took time to develop.  With CRISPR, on the other hand, all people need to do is synthesize a guide RNA (gRNA) that binds to a specific DNA sequence and the CRISPR/Cas9 will do its job.

However, just like earlier genome editing techniques, CRISPR is used to created double stranded breaks at a target sequence.  This can be used to knockout genes, introduce transgenes through the homology-directed repair pathway, or repair defective copies of genes by supplying a normal copy of the gene that gets incorporated through the HDR mechanism.  There's a few other uses for it, but those three are generally the most common uses. 

Like I said before, this technology is in its infancy and still has many issues.  One of the biggest issues is that CRISPR/Cas9 has a greater likelihood of off-target effects opposed to TALENs and ZFNs.  There was an article published in 2015, and was mentioned late in the first video, by scientists in china who used CRISPR/Cas9 on tripronuclear (3PN) human zygotes to see if they could effectively repair a defective copy of a certain gene.  However, the frequency of homologous recombination of their supplied transcript was low, roughly 25% (off the top of my head).  Even then, there were issues of CRISPR/Cas9 having off-target endonuclease activity on a homolog for the gene, which they were unable to resolve.

I think the first video really over-glorifies the capabilities of CRISPR/Cas9 as it is now, but I am rather interested in its gene drive implications that appear to lead to "super-mendelian inheritance".  There is also research into combining the sequence targeting capabilities of CRISPR with enzymatic domains of adenosine deaminases to create base editors that appear to have more effective use compared to the standard CRISPR/Cas9 system.

Also, no TL;DR, you lazy fucks.

88
The Flood / Re: Who's better looking: Jester or Class
« on: September 22, 2016, 06:20:11 AM »
Jester looks like he would shoot up a school and class kinda looks like an alien with his small face but large eyes.  Ayy lmao.

89
The Flood / Re: Do you guys have users you don't take seriously?
« on: August 06, 2016, 08:22:15 PM »
SecondClass because he is a nu-male cuck apparently.

90
The Flood / Re: How come women aren't called out for fat shaming men?
« on: August 06, 2016, 02:39:03 PM »
I'm still trying to figure out how people can maintain being over 300+ lbs.  Like, just how much crap are people eating to be like 300+ lbs?  The biggest I ever got to was 210 lbs, but I've gotten myself down to 153 lbs.  Honestly, people these days don't like to take responsibility for their actions.  This is why people always claim "muh genetics" when in reality they are just fucking pigs.  Do people not realize how easy it is to lose weight when they stop drinking soda?

The biggest I've been is 240. I really don't see how it's possible to hit 300 because I was eating absolute garbage when I was at 240 and I just stopped gaining weight.

Maybe there is something to muh genetics after all.
No, not really.  It's just people overeating.  Some researchers tested some mice that were ob/ob, meaning they had homozygous mutation that promoted obesity.  What they found was that a hypoediting of a sodium channel transcript at one specific location led to the mice overeating.  In an environment where the mice could at ad libitum, the mice became obese.  However, when the eating habits of the mice were controlled, they were a normal weight compared to normal mice under the same regimen.  In other words, fatties are lazy fucks that don't know what self-control is and want to blame other people for their faults.

Dang, but what about smoking cessation's effects on the body?
Idk, haven't really read any research papers on that stuff.

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