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Messages - Blonic the Fluxmeister
61
« on: December 28, 2016, 05:28:08 PM »
Google Pixel for Christmas. Camera is much better than the crappy phone I had before this one.
62
« on: December 28, 2016, 05:12:59 PM »
calc 2 - C
anthro - A
multcultural education - B
astrophysics - B
uni physics 1 - B
overall i got a 2.94 gpa which is dumb
Looks like someone is going over the wall.
63
« on: December 28, 2016, 01:56:11 PM »
Ended up getting a 3.93. Could've had a 4.0 if I didn't fuck up one exam in one of my classes.
64
« on: December 27, 2016, 11:08:21 AM »
So is ONI destabilizing the Middle East?
65
« on: December 27, 2016, 10:58:56 AM »
White privilege is a buzzword invented to disrupt discussion. It's pretty sad tbh.
"Oh, you're white, therefore anything you say is invalid and I automatically win the argument. *furious snapping*"
66
« on: November 27, 2016, 07:52:32 PM »
This is now a "We are Number One" thread.
kill yourself my man
literal fucking piece of shit
67
« on: November 27, 2016, 12:33:00 PM »
This is now a "We are Number One" thread.
68
« on: November 27, 2016, 01:20:55 AM »
Is this the sperg out I heard about?
What sperg out? The fuck is going on?
Class kicked everyone, and was the only mod online at the time it happened, and is now poorly trying to make it seem like he did nothing and that everyone should join the server where he is now admin to sate his ego among other things I imagine.
this never happened but ok
Gonna need to see some proofs for that.
proof I didn't do something lmao
People have claims that you kicked everyone. The burden of proof rests on you.
69
« on: November 27, 2016, 01:15:06 AM »
Is this the sperg out I heard about?
What sperg out? The fuck is going on?
Class kicked everyone, and was the only mod online at the time it happened, and is now poorly trying to make it seem like he did nothing and that everyone should join the server where he is now admin to sate his ego among other things I imagine.
this never happened but ok
Gonna need to see some proofs for that.
70
« on: November 25, 2016, 10:49:56 AM »
So...you don't like good music?
71
« on: November 22, 2016, 09:52:48 AM »
Is this the Sep7 one or the nazi one
Heil fucking Hitler.
72
« on: November 22, 2016, 09:51:56 AM »
So you aren't as vehement about trump and right-leaning individuals as you made yourself out to be in the discord chats?
I'm confuzzled.
73
« on: November 17, 2016, 03:39:06 PM »
You're just one year closer to your death, thank god.
74
« on: November 11, 2016, 05:27:51 PM »
>What is the checks and balances system.
What is Aleppo?
Leppo my eppo?
75
« on: November 11, 2016, 05:21:23 PM »
>What is the checks and balances system.
76
« on: November 09, 2016, 12:42:46 AM »
Pennsylvania was called for Trump. It's official, Trump wins.
77
« on: November 09, 2016, 12:20:10 AM »
I bet SecondClass is crying as he watches Clinton get fucked.
78
« on: October 31, 2016, 10:11:40 PM »
Don't forget the video of the DNC chairman admitting that voter fraud occurs, especially from the left.
79
« on: October 31, 2016, 09:28:31 PM »
Voting for Trump. :3
80
« on: October 11, 2016, 07:13:26 AM »
ITT: Flee is fukt.
81
« on: October 09, 2016, 09:15:23 PM »
Talk about a biased moderator.
82
« on: October 01, 2016, 08:19:48 PM »
Addictinggames.com was my childhood, so fuck off.
83
« on: October 01, 2016, 12:59:50 PM »
it's rampant in our society and desperately needs to be stopped
I know right? Muslims are so very anti-semitic.
84
« on: October 01, 2016, 12:57:48 PM »
>he doesn't get high off of Flintstone's gummies
sheeeeeeit
85
« on: September 27, 2016, 05:22:56 PM »
Go take your stupid Rubicon elsewhere, Chakas. The Lifeshaper isn't here.
86
« on: September 27, 2016, 03:00:11 PM »
hashtag dadbod
I'll still fuck up every single one of you in a fight.
Except maybe Jive 'cause he's a juicing ass nigga now
I think that stash makes you look like a pedo.
87
« on: September 27, 2016, 06:27:18 AM »
By the way, if anyone is actually interested in reading some good review/research articles on CRISPR, I'm happy to provide them.
88
« on: September 26, 2016, 12:30:20 PM »
There was an article published in 2015, and was mentioned late in the first video, by scientists in china who used CRISPR/Cas9 on tripronuclear (3PN) human zygotes to see if they could effectively repair a defective copy of a certain gene. However, the frequency of homologous recombination of their supplied transcript was low, roughly 25% (off the top of my head).
Is 25% success higher than what we're currently getting with ZFN or TALEN methods? If not, then the main advantage of CRISPR is saving time and funding to produce at least some results (I'm thinking a quick brute-force method over meticulous but productive).
I think the first video really over-glorifies the capabilities of CRISPR/Cas9 as it is now, but I am rather interested in its gene drive implications that appear to lead to "super-mendelian inheritance". There is also research into combining the sequence targeting capabilities of CRISPR with enzymatic domains of adenosine deaminases to create base editors that appear to have more effective use compared to the standard CRISPR/Cas9 system.
I kinda got that vibe too from it, but if it's something that could be spectacular in it's infancy then it's worth waiting to see what comes of it as it's researched and tested more (particularly if the upfront cost is lower).
Who knows, with gene-drive it could be possible to eradicate other diseases by targeting the parents without having to genetically alter a zygote/fetus during pregnancy and all the risks that brings (but I'm completely out of my element here, so I don't know how exactly that would work, if at all).
From what I know, I don't think anyone has tried using ZFNs and TALENs in human embryos like that china paper, considering that gene editing in humans is a rather controversial topic. Maybe there are other papers that have done a similar approach with these other technologies, but I'm not entirely sure. Most research using these technologies has been in other model organisms. And yes, I agree with the idea that is can be a very innovative technology down the road, but I felt that the video was overblowing its current capabilities and really tending to shrug off the technical issues that CRISPR faces. CRISPR is definitely a cheaper and faster method for genome editing, but it also lacks the specificity of ZFNs and TALENs.
89
« on: September 26, 2016, 11:01:52 AM »
It's gonna be quite a while for sure before we really see full-fledged genome editing in humans. The CRISPR/Cas9 technology, which has been adopted from bacteria, is still very much in its infancy and has many complications. The reason why it is heralded as being such a game changer is that this technology functions much differently as opposed to older genome editing technologies like Zinc-Finger Nucleases (ZFNs and Transcription activator-like effector nucleases (TALENs). These earlier technologies involved altering the binding specificity of the DNA-binding domains to bind to specific DNA sequences, which took time to develop. With CRISPR, on the other hand, all people need to do is synthesize a guide RNA (gRNA) that binds to a specific DNA sequence and the CRISPR/Cas9 will do its job.
However, just like earlier genome editing techniques, CRISPR is used to created double stranded breaks at a target sequence. This can be used to knockout genes, introduce transgenes through the homology-directed repair pathway, or repair defective copies of genes by supplying a normal copy of the gene that gets incorporated through the HDR mechanism. There's a few other uses for it, but those three are generally the most common uses.
Like I said before, this technology is in its infancy and still has many issues. One of the biggest issues is that CRISPR/Cas9 has a greater likelihood of off-target effects opposed to TALENs and ZFNs. There was an article published in 2015, and was mentioned late in the first video, by scientists in china who used CRISPR/Cas9 on tripronuclear (3PN) human zygotes to see if they could effectively repair a defective copy of a certain gene. However, the frequency of homologous recombination of their supplied transcript was low, roughly 25% (off the top of my head). Even then, there were issues of CRISPR/Cas9 having off-target endonuclease activity on a homolog for the gene, which they were unable to resolve.
I think the first video really over-glorifies the capabilities of CRISPR/Cas9 as it is now, but I am rather interested in its gene drive implications that appear to lead to "super-mendelian inheritance". There is also research into combining the sequence targeting capabilities of CRISPR with enzymatic domains of adenosine deaminases to create base editors that appear to have more effective use compared to the standard CRISPR/Cas9 system.
Also, no TL;DR, you lazy fucks.
90
« on: September 22, 2016, 06:20:11 AM »
Jester looks like he would shoot up a school and class kinda looks like an alien with his small face but large eyes. Ayy lmao.
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